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Antibodies for Immuno-Oncology Research

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Rabbit anti-Glu-Glu Antibody HRP Conjugated - A190-111P - 0.1 mg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Glu-Glu Antibody HRP Conjugated
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Size: 0.1 mg
£POA
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Catalogue number:
A190-111P
Name:
Rabbit anti-Glu-Glu Antibody HRP Conjugated
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Rabbits were immunized with a synthetic peptide representing amino acid residues 314 - 319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP).Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1.

Applications:

WB, IHC, ICC, ELISA

Product Type:
Antibodies
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of Glu-Glu-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, rabbit anti-Glu-Glu antibody A190-111P used for WB at 0.2 µg/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 3 minutes.

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