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Goat anti-Glu-Glu Antibody HRP Conjugated

Catalogue number:
A190-110P
Size:
0.1 mg
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Goats were immunized with a synthetic peptide representing amino acid residues 314 - 319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP).Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1.

  • Applications: WB, IHC, ICC, ELISA
Product Type:

Antibodies

Storage Temp:

2 - 8°C

Antibody Host:

Goat

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of Glu-Glu-tagged Protein by w

Detection of Glu-Glu-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, goat anti-Glu-Glu antibody A190-110P used for WB at 0.2 µg/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 30 seconds.