Goats were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1.
avian myelocytomatosis viral oncogene homolog, bHLHe39, class E basic helix-loop-helix protein 39, c-Myc, MRTL, myc proto-oncogene protein, MYCC, myc-related translation/localization regulatory factor, proto-oncogene c-Myc, transcription factor p64, v-myc
2 - 8°C
Detection of c-myc-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, goat anti-c-myc antibody A190-104P used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 10 seconds.