Chickens were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to fluorescein isothiocyanate (FITC). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
avian myelocytomatosis viral oncogene homolog, bHLHe39, class E basic helix-loop-helix protein 39, c-Myc, MRTL, myc proto-oncogene protein, MYCC, myc-related translation/localization regulatory factor, proto-oncogene c-Myc, transcription factor p64, v-myc
2 - 8°C
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide