Chicken anti-c-myc Antibody Biotinylated - A190-103B - 0.1 mg - Bethyl Laboratories, Inc. - antibodies
Chickens were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to biotin.Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, ELISA
Detection of c-myc-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, chicken anti-c-myc antibody A190-103B used for WB at 0.04 µg/ml (1:25,000). Detection: Streptavidin-HRP (0.1 µg/ml) and chemiluminescence with an exposure time of 10 seconds.
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