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Chicken anti-Myc Tag Antibody Biotinylated

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Applications:

WB, ELISA

Antibody Host:

Chicken

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

Alternative Names:

avian myelocytomatosis viral oncogene homolog, bHLHe39, class E basic helix-loop-helix protein 39, c-Myc, MRTL, myc proto-oncogene protein, MYCC, myc-related translation/localization regulatory factor, proto-oncogene c-Myc, transcription factor p64, v-myc

Product Description:

Chickens were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to biotin. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage Temperature:

2 - 8°C

Detection of c-myc-tagged Protein by wes
Detection of c-myc-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, chicken anti-c-myc antibody A190-103B used for WB at 0.04 µg/ml (1:25,000). Detection: Streptavidin-HRP (0.1 µg/ml) and chemiluminescence with an exposure time of 10 seconds.
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