Goats were immunized with ECS, Enterokinase Cleavage Site (xxxDDDDK) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to fluorescein isothiocyanate (FITC). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By ELISA and Western blot, this antibody reacts with FLAGTM.
- Applications: IHC, ICC, IF
2 - 8°C