The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1. By immunoelectrophoresis and ELISA this antibody reacts specifically with Bat IgG and with light chains common to other Bat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Antibody has been shown to react with bat genus species Pteropus vampirus, Desmodus rotundus, Eptesicus fuscus, Tadrida pumila, T. condylura, Hypsignathus monstrosus, Rosettus aegyptiacus, Epomorphus crypturus, Molossus species, and Phylostomus species. This antibody may cross react with IgG from other species.
- Applications: WB, IHC, ICC, ELISA
2 - 8°C