Goats were immunized with purified IgG from dove, duck, sparrow and chicken serum. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with dove, duck, sparrow and chicken bird IgG. Turkey, emu, ostrich, quail, macaw and cockateil IgG are also detected. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
- Applications: IHC, ICC, F, IF
2 - 8°C