The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to alkaline phosphatase (alkphos). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme: antibody protein ratio is 1:1. By immunoelectrophoresis and ELISA this antibody reacts specifically with monkey IgG and with light chains common to other monkey immunoglobulins. Species detected are Rhesus, Baboon, Carb Green, Cyno, Chimp and African Green. Reaction to human was also detected. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
2 - 8°C