Rabbit anti-Sheep IgM Antibody HRP Conjugated

Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1. By immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgM. This antibody may cross react with IgM from other species.

• Applications: WB, IHC, ICC, ELISA