Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1. By immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgA. This antibody may cross react with IgA from other species.
- Applications: WB, IHC, ICC, ELISA
2 - 8°C