Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis, this antibody reacts specifically with Rabbit IgG-Fc Fragment. This antibody may cross react with IgG from other species.
2 - 8°C
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide