Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to alkaline phosphatase (alkphos). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme: antibody protein ratio is 1:1. By immunoelectrophoresis, this antibody reacts specifically with rabbit IgG. This antibody may cross react with IgG from other species.
2 - 8°C
50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA and 0.09% NaN3