Goat anti-Rabbit IgG-heavy and light chain Antibody Biotinylated

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to biotin. Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Biotin/antibody protein ratio is 4:1. By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. Biotinylated antibody was demonstrated by reaction with avidin/peroxidase when coated on microtiter wells. Working dilutions should be determined by the investigator.

• Applications: WB, IHC, ICC, ELISA