The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to alkaline phosphatase (alkphos). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme: antibody protein ratio is 1:1. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
- Applications: WB, IHC, ICC, ELISA
2 - 8°C