Antiserum was cross adsorbed using human, mouse and pig immunosorbents to remove cross reactive Antibodies. The antibody to bovine albumin was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme:antibody protein ratio is 1:1. By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine albumin. Less than 0.1% cross reactivity to human, mouse and pig albumin was detected. This antibody may cross react with albumin from other species.
- Applications: WB, IHC, ICC, ELISA
2 - 8°C