Antiserum was solid phase absorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ration is 4:1. By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine igG2. This antibody may cross react with IgG2 from other species.
2 - 8°C
Phosphate Buffered Saline (PBS) containing 0.05% Pro-Clean 400