Sheep anti-Bovine IgG-heavy and light chain Antibody Alkaline Phosphatase Conjugated - A10-115AP - 1 mg - Bethyl Laboratories, Inc. - antibodies

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to alkaline phosphatase (alkphos). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme:antibody protein ratio is 1:1. By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine IgG and with light chains common to other bovine immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

Applications:

WB, IHC, ICC, ELISA