Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to pig IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to alkaline phosphatase (alkphos). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with pig IgG and with light chains common to other pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, horse, human, mouse, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
- Applications: WB, IHC, ICC, ELISA
2 - 8°C