Maleimides are among the most frequently used reagents for thiol modification. In most proteins, the sites of reactions are at cysteine residues that either are intrinsically present or result from reduction of cystines. Unlike iodoacetamides, maleimides do not react with histidines and methionines under physiological conditions. Tetramethylrhodamine-5-maleimide is widely used for thiol modifications of peptides and proteins.
Reagents & Kit Components
-20°C desiccated and protected from light
1. Graceffa P and Dominguez R (2003). J Biol Chem 278, 34172-80. 2. Tong Y, et al. (2001). J Gen Physiol 117, 103-18.