HiLyte™ Fluor 488 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates far superior to those of fluorescein derivatives such as FITC. Not only are HiLyte™ Fluor 488 conjugates significantly brighter than fluorescein conjugates, they are much more photostable. Additionally the fluorescence of HiLyte™ Fluor 488 fluorophore is independent of pH from 4 to 10. This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only above pH 10.
Reagents & Kit Components
-20°C desiccated and protected from light