SensoLyte® 440 Cathepsin S Assay Kit *Fluorimetric* - ANA72100 - 1 kit - AnaSpec - assays & kits

Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin S is a cysteine proteinase involved in the pathogenesis of autoimmune diseases, atherosclerosis,Cancer, obesity and related diseases. The SensoLyte® 440 Cathepsin S Assay Kit uses a fluorogenic peptide for measurement ofEnzyme activity. This peptide releases the AMC (7-amino-4-methylcoumarin) fluorophore upon cathepsin S cleavage, and which can be detected with excitation at 354 nm and emission at 442 nm. The kit contains: , AMC based fluorogenic substrate (Ex/Em=354 nm/442 nm upon cleavage) , Assay buffer , Human spleen Cathepsin S , Inhibitor , Fluorescence reference standard for calibration , A detailed protocol