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SensoLyte® 390 Cathepsin D Assay Kit *Fluorimetric*

Supplier:
Catalogue number:
ANA72098
Size:
1 kit
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£601.00 Shipping is calculated in checkout

Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. It is involved in the pathogenesis of several diseases such as breastCancer and Alzheimer disease. The SensoLyte® 390 Cathepsin D Activity Assay Kit uses a Mca/Dnp FRET peptide for measurement ofEnzyme activity. In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin D, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330 nm/390 nm. The kit contains: , Mca/Dnp-based FRET peptide substrate (Ex/Em=330 nm/390 nm upon cleavage) , Assay buffer , Bovine spleen Cathepsin D , Inhibitor , Fluorescence reference standard for calibration , A detailed protocol

Product Type:

Assays & Kits

Storage Temp:

-20°C

UNSPSC Code:

41116012