HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy® 3 dyes, resulting in an optimal match to filters designed for Cy® dyes. The HiLyte™ Fluor 555-protein conjugates are very stable and suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. Additionally, our in-house data indicated that HiLyte™ Fluor 555 is superior to Cy® 3 in fluorescence polarization-based assays. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte™ Fluor 555-protein conjugate. The entire process takes only half an hour. The kit is able to perform three conjugation reactions with each reaction labeling up to 200 µg IgG. Kit Size: 3 labeling reactions (3 x 200 µg protein).
Note: AnaTag kits are not intended for peptide labeling.
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