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SensoLyte® 520 MMP - 14 Assay Kit *Fluorimetric*

Supplier:
Catalogue number:
ANA72025
Size:
1 kit
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£665.00 Shipping is calculated in checkout

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-14 (MT1-MMP) is the membrane bound MMP. When expressed in cells, the catalytic and the hemopexin domains of MMP-14 become exposed on cell surfaces. ThisEnzyme plays an important role in tumor invasion. SensoLyte® 520 MMP-14 Assay Kit uses a 5-FAM (fluorophore) and QXL®520 (quencher) labeled FRET peptide substrates for continuous measurement of theEnzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon the cleavage of the FRET peptide by MMP-14, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL®520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Product Type:

Assays & Kits

Storage Temp:

-20°C

UNSPSC Code:

41116012