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3D Collagen Matrix Cell Contraction Assay

Highly effective & efficient in vitro delivery of Cas9 RNP for genome editing in challenging cells

SensoLyte® 520 MMP - 10 Assay Kit *Fluorimetric* - ANA72024 - 1 kit - AnaSpec - assays & kits

SensoLyte® 520 MMP - 10 Assay Kit *Fluorimetric*
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Size: 1 kit
£650.00
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Catalogue number:
ANA72024
Name:
SensoLyte® 520 MMP - 10 Assay Kit *Fluorimetric*
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MMP-10 (stromelysin 2) cleaves extracellular matrix proteins, but is unable to cleave the triple-helical fibrillar collagens. ThisEnzyme is involved in several pathological conditions, such asCancer, arthritis and wound healing. The SensoLyte® 520 MMP-10 Assay Kit uses a 5-FAM (fluorophore) and QXL®520 (quencher) labeled FRET peptide substrates for continuous measurement of theEnzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon the cleavage of the FRET peptide by MMP-10, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL®520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

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Product Type:
Assays & Kits
Storage Temp:
-20°C
UNSPSC Code:
41116012
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