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Sensolyte® Cell Viability and Proliferation Assay Kit

Supplier:
Catalogue number:
ANA71300
Size:
1 kit
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Lactate dehydrogenase (LDH) exists ubiquitously in the cytoplasm of living mammalian cells and the measurement of cytoplasmic LDH activity is a well-accepted assay to quantify living cell numbers and indicate cell viablity. The SensoLyte® Cell Viability and Proliferation Assay Kit provides researchers with a convenient one-solution and one-step assay to count living cells in a culture and continuously monitor cell proliferation over time by measuring cytoplasmic LDH activity. In this kit, resazurin is used as a sensitive fluorogenic indicator. Resazurin is converted to the strongly fluorescent resorufin (Ex/Em=560nm/590 nm) by cytoplasmic LDH. The kit can detect as few as 48 living cells with a linear range up to 5X104 cells (R2=0.99). This kit can also be used for high throughput screening of cell proliferation or measuring cytotoxicity effect of a variety of compounds. 384-well or 1536-well format can be used with minor modifications.

Product Type:

Assays & Kits

Storage Temp:

-20°C

UNSPSC Code:

41116111