Protein phosphatases have received great attentions as drug-screening targets. p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for protein tyrosine phosphatases and serine/threonine phosphatases. SensoLyte® pNPP Protein Phosphatase Assay Kit uses pNNP to quantify protein phosphatase activities. The kit can be used for characterizing kinetics ofEnzyme reaction and high throughput screening of protein phosphatase inhibitors. It has high sensitivity and wide linear range (The detection limit is generally 3 ng or below). The kit contains:pNNP chromogenic substrate, Protein phosphatase inhibitor, Assay buffer, A 'mix and read' assay protocol that is compatible with HTS liquid handling instruments
Biochemical and cellular assays
Ref: Urbanek RA et al (2001) Potent reversible inhibitors of the protein tyrosine phosphatase CD45. J Med Chem. 44, 1777-93; Marley AE et al (1996) Biochemical characterization and deletion analysis of recombinant human protein phosphatase 2C alpha. Biochem J. 320, 801-6.