This novel fluorogenic substrate of HDACs was synthesized with an epsilon-acetylated lysyl moiety and an adjacent MCA moiety at the C-terminus of the peptide chain. The assay utilizing this substrate provides a good tool to characterize the HDAC activity. HDACs are importantEnzymes for the transcriptional regulation of gene expression.
Proteins & Peptides
Peak Area by HPLC ≥95%
Wegener, D. et al. Chem. Biol. 10, 61 (2003).