MMP Colorimetric Substrate I - ANA27097 - 5 mg - AnaSpec - proteins & peptides
MMP Colorimetric Substrate I
Catalogue number:
ANA27097
Name:
MMP Colorimetric Substrate I
Supplier:
Availability:
An exceptional MMP-2 (gelatinase A) and MMP-9 (gelatinase B) substrate this peptide is used for the continuous spectrophotometric assay of MMP-2 and MMP-9 in combination with a color-developing thiol-reactive agent, 4,4’-dithiodipyridine or Ellman’s Reagent (as indicators). This thiol peptide-enzyme reaction has a Km of 0.004 M and a kcat of 103 s-1. Using this substrate, collagenase can be detected at concentrations as low as 2 ng/mL. HPLC and tandem mass spectrometry are also used to analyze MMP-2 and MMP-9 in conjunction with this peptide substrate.
Data sheet:
Product Type:
Proteins & Peptides
Alternative Names:
Ac-PLG-SCH[CH2CH(CH3)2]-CO-LG-OC2H5
Storage Temp:
-20°C
Compound Purity:
Peak Area by HPLC ≥95%
Molecular Weight:
655.9
Reference:
Ref: Weingarten, H. and J. Feder, Anal. Biochem. 147, 437 (1985); Weingarten, H. et al. Biochem. 24, 6730 (1985).
UNSPSC Code:
12352202
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