Tel: +44 (0)1223 316 855
life science research products, biological research products, biotechnology
close ...


Supplier:
Product types:
Find Your Peptide Simply with Our Peptide Search
Highly Bright & Stable Bright Chemi Substrates
Over 40,000 Peptides & Proteins Available

Upgrading Your Laser Scanner? Consider The Sapphire™

360 MMP FRET Substrate III - ANA27094 - 1 mg - AnaSpec - proteins & peptides

360 MMP FRET Substrate III
print page
Catalogue number:
ANA27094
Name:
360 MMP FRET Substrate III
Supplier:
Availability:
This product is no longer available to order.

Optimized for both 72-kDa fibroblast gelatinase(MMP 2) and 92-kDa neutrophil gelatinase(MMP 9), this peptide substrate has the fluorescent Trp residue in subsite P’2 and the dinitrophenol (Dnp) quenching group on the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-P’1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-P’1 and P’3 are optimized for MMP, while Arg is placed in P’4 to enhance solubility. The water solubility of this peptide substrate in assay buffer exceeds the Km value for the enzyme reaction, allowing accurate determination of the kinetic parameters. Abs/Em = 280/360 nm.

Data sheet:

Click to view / download

Product Type:
Proteins & Peptides
Alternative Names:
Dnp-PLGMWSR
Storage Temp:
-20°C
Compound Purity:
Peak Area by HPLC ≥95%
Molecular Weight:
1012.1
Reference:
Ref: d’Ortho, MP. et al. Eur. J. Biochem. 250, 751 (1997); Netzel-Arnett, S. et al. Anal. Biochem. 195, 86 (1991).
UNSPSC Code:
12352202
Contact Our Friendly Technical Team