EDANS is one of the most popular donors for developing FRET-based nucleic acid probes and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive.
Reagents & Kit Components
-20°C desiccated and protected from light
Becker BF, et al. (2002). Application of peptides containing the cleavage sequence of pro-TNFa in assessing TACE activity of whole cells. Biol Chem 383, 1821-6; Wang GT, et al. (1993). A continuous fluorescence assay of renin activity. Anal Biochem 210, 351-9.