EDANS is one of the most popular donors for developing FRET-based nucleic acid probes and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive.
Reagents & Kit Components
-20°C desiccated and protected from light
Ref: Becker BF, et al. (2002). Application of peptides containing the cleavage sequence of pro-TNFalpha in assessing TACE activity of whole cells. Biol. Chem 383, 1821-6; Cottaz S, et al. (2000). A fluorescence-quenched chitopentaose for the study of endo-chitinases and chitobiosidases. Eur J Biochem 267, 5593-600; Beekman B, et al. (1996). Convenient fluorometric assay for matrix metalloproteinase activity and its application in Biol.ogical media. FEBS Lett 390, 221-5; Wang GT, et al. (1993). A continuous fluorescence assay of renin activity. Anal Biochem 210, 351-9.