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Her2 (ERBB2) Mouse Monoclonal Antibody [Clone ID: UMAB36]

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UM500036
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
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Applications:

10k-ChIP, IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human, Monkey, Mouse, Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

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Product Description:

Purified HER2 (ERBB2) mouse monoclonal antibody,clone UMAB36

Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ERBB2-Knockout hela cells (KO, Cat# LC810070) were separated by SDS-PAGE and immunoblotted with anti-ERBB2 monoclonal antibody UM500036. Then the blotted membrane was stripped and reprobed with anti-β-actin (TA811000) as a loading control (1:500).
OriGene overexpression protein microarra
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERBB2 mouse monoclonal antibody (Clone UMAB36). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-ERBB2 (Clone UMAB36) very specifically recognizes ERBB2 antigen on OriGene protein microarray chip.
Anti-ERBB2 mouse monoclonal antibody (UM
Anti-ERBB2 mouse monoclonal antibody (UM500036, clone UMAB36, 1:100) immunofluorescent staining of MCF7 cells.
Anti-ERBB2 mouse monoclonal antibody (UM
Anti-ERBB2 mouse monoclonal antibody (UM500036, clone UMAB36, 1:100) immunofluorescent staining of COLO205 cells.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of breast tissue (HER2+++; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) using anti-ERBB2 mouse monoclonal antibody. (Clone UMAB36, dilution 1:100; heat-induced epit
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERBB2mouse monoclonal antibody. (Clone UMAB36, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Western blot analysis of cell lysates of
Western blot analysis of cell lysates of HEK293 transfected with ERBB2 cDNA (Left lane) or untransfected (Right lane) by using ERBB2 UltraMAB (UM500036, clone UMAB36, 1:2000).
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERBB2 monoclonal antibody (Clone UMAB36) at 1:500.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ERBB2 monoclonal antibody (1:500).
Western blot of human tissue lysates (15
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
Immunohistochemical staining of four par
Immunohistochemical staining of four paraffin-embedded 293T cell lines transfected with EGFR, ERBB2, ERBB3, or ERBB4 using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM500036) (1:500). The image shows only ERBB2 transfected cell line lights up with anti-ERBB2 clone UMAB36 showing no cross-reactivity to the other EGFR family members.
Immunohistochemical staining of FFPE HER
Immunohistochemical staining of FFPE HER2+ breast cancer; using heat-induced epitope retrieval HIER at 120°C for 3min with TEE buffer pH9.0, mouse monoclonal antibody anti-ERBB2 clone UMAB36 was used at 1ug/mL. Strong membrane staining seen in tumor cells.
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