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CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: UMAB32]

Catalogue number:
Supplier:
Size:
100 µl _$$_
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Applications:

10k-ChIP, FC, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

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Product Description:

PECAM1 mouse monoclonal antibody,clone UMAB32

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1 mouse monoclonal antibody.1:500`
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PECAM1 monoclonal antibody (Clone UMAB32) at 1:500.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 8 different human tissues by using anti-PECAM1 monoclonal antibody. 1:250
Western Blot analysis of 10 different hu
Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-PECAM1 monoclonal antibody (clone UMAB32, 1:500)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB32, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB32, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB32, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB32, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB32, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB32, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB32, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded lymph node tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB32, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded human placenta using anti-PECAM clone UMAB32 mouse monoclonal antibody (UM500032) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membraneous staining is seen in the endothelia cells and weak staining in the trophoblast.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded human skin using anti-PECAM clone UMAB32 mouse monoclonal antibody (UM500032) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membraneous staining is seen in the vasular endothelia cells.
Living HEK293T cells transfected with ei
Living HEK293T cells transfected with either RC208654 plasmid (red) or empty vector (blue) were immunostained by anti-PECAM1 antibody (UM500032) or isotype control antibody (green), and then analyzed by flow cytometry (1:100).
OriGene overexpression protein microarra
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PECAM1 mouse monoclonal antibody (Clone UMAB32). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-PECAM1 (Clone UMAB32) very specifically recognizes PECAM1 antigen on OriGene protein microarray chip.
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