ERCC1 Mouse Monoclonal Antibody [Clone ID: 2E12]

Catalogue number:

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UM500011

Supplier:

OriGene

Size:

100 µl _$$_

Product is available in:

N/A

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Applications:

10k-ChIP, IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human, Monkey, Mouse, Rat

Antibody Isotype:

IgG2b

Antibody Type:

Monoclonal

Data sheet: View or download
MSDS: View or download

Product Description:

ERCC1 mouse monoclonal antibody, clone 2E12

Western blot analysis of extracts (35ug) — Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody (Clone 2E12).

Western Blot analysis of 10 different hu — Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-ERCC1 monoclonal antibody (clone 2E12, 1:500)

Equivalent amounts of cell lysates (10 u — Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and ERCC1-Knockout Hela cells (KO, Cat# LC810072) were separated by SDS-PAGE and immunoblotted with anti-ERCC1 monoclonal antibody UM500011. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin — Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunofluorescent staining of HeLa cells — Immunofluorescent staining of HeLa cells using ERCC1 mouse monoclonal antibody (UM500011, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

OriGene overexpression protein microarra — OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERCC1 mouse monoclonal antibody (Clone 2E12). The positive reactive proteins are highlighted with red arrows in the enlarged subarray. Other positive controls spotted in this subarray are serial dilutions of mouse IgG as controls.

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