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CD5 Mouse Monoclonal Antibody [Clone ID: UMAB9]

Catalogue number:
Supplier:
Size:
100 µl _$$_
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Applications:

FC, IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

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Product Description:

CD5 mouse monoclonal antibody, clone UMAB9

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5 (1:500).
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CD5 monoclonal antibody (1:500).
Western Blot analysis of 10 different hu
Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-CD5 monoclonal antibody (clone UMAB9, 1:500)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD5 clone UMAB9 mouse monoclonal antibody at 1:200 dilution of 0.1mg/mL detection with Polink2 Broad HRP DAB (UM500009 requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in >75 % of non germinal center cells of tonsil and
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM500009) (1:300).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded mouse thymus tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM500009) (1:300).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM500009) (1:300).
Immunofluorescent staining of Jurkat cel
Immunofluorescent staining of Jurkat cells using CD5 mouse monoclonal antibody (UM500009, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Flow cytometric Analysis of Jurkat cells
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody (UM500009), (Red), compared to a nonspecific negative control antibody, (Blue).
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