ERCC1 Mouse Monoclonal Antibody [Clone ID: 4F9]

Catalogue number:

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UM500008

Supplier:

OriGene

Size:

100 µl _$$_

Product is available in:

N/A

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Applications:

10k-ChIP, IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human, Mouse

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Data sheet: View or download
MSDS: View or download

Product Description:

Anti-ERCC1 mouse monoclonal antibody, clone 4F9

OriGene overexpression protein microarra — OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERCC1 mouse monoclonal antibody (Clone 4F9). The positive reactive proteins are highlighted with red arrows in the enlarged subarray. Other positive controls spotted in this subarray are serial dilutions of mouse IgG as controls.

Western blot analysis of extracts (35ug) — Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody (Clone 4F9).

Western blot of human tissue lysates (15 — Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Equivalent amounts of cell lysates (10 u — Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and ERCC1-Knockout Hela cells (KO, Cat# LC810072) were separated by SDS-PAGE and immunoblotted with anti-ERCC1 monoclonal antibody UM500008. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

Immunohistochemical staining of FFPE nor — Immunohistochemical staining of FFPE normal adjacent lung from tumor specimen using heat-induced epitope retrieval HIER at 120°C for 3min with Accel buffer pH8.7, mouse monoclonal antibody anti-ERCC1 clone 4F9 was used at 1ug/mL. Strong nuclear stain in lung pneumocytes and macrophages.

Immunohistochemical staining of FFPE end — Immunohistochemical staining of FFPE endometrial carcinoma using heat-induced epitope retrieval HIER at 120°C for 3min with Accel buffer pH8.7, mouse monoclonal antibody anti-ERCC1 clone 4F9 was used at 1ug/mL. Strong nuclear stain in tumor cells.

Immunofluorescent staining of HeLa cells — Immunofluorescent staining of HeLa cells using anti-ERCC1 mouse monoclonal antibody (UM500008, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

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