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BubR1 (BUB1B) Mouse Monoclonal Antibody [Clone ID: UMAB7]

Catalogue number:
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UM500007
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human, Monkey

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

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Product Description:

Anti-BUB1B mouse monoclonal antibody, clone UMAB7

Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody (Clone UMAB7) at 1:500.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (Clone UMAB7, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded human endometrial carcinoma using anti-BUB1B a mouse monoclonal antibody. Heat-induced epitope retrieval by ACCEL (pH8.7) in pressure chamber/cooker at 110°C for 3min, UM500007 clone UMAB7 at 1:250. Images shows tumor cells with strong cytoplasmic staining.
Immunofluorescent staining of A549 cells
Immunofluorescent staining of A549 cells using anti-BUB1B mouse monoclonal antibody (UM500007, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Immunofluorescent staining of HeLa cells
Immunofluorescent staining of HeLa cells using anti-BUB1B mouse monoclonal antibody (UM500007, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
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