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Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: UMAB3]

Catalogue number:
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UM500003
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

10k-ChIP, IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human, Dog

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

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Product Description:

KRT19/CK19 mouse monoclonal antibody,clone UMAB3

Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CK19 monoclonal antibody (Clone UMAB3) at 1:500.
HEK293T were transfected with 55 differe
HEK293T were transfected with 55 different plasmids of CK cDNA (1, 2, 4, 5, 6a, 6b, 6c, 7, 8, 9, 12, 13, 14, 15, 16, 17, 18 v1, 18 v2, 19, 20, 24, 25, 26, 27, 28, 31, 32, 33a, 33b, 34, 35, 36, 37, 38, 39, 40, 71, 72 v1, 72 v3, 73, 74, 75, 76, 77, 78, 79, 80 v1, 80 v2, 81, 82, 83, 84, 85, 86 and 222) for 48hrs and lysed. Cell lysates (5 ug per lane) were separated by SDS-PAGE and blotted with KRT19 antibody. KRT12, 19, 25, 26, 27, 28 and 39 were positive, while all others were negative (1:500).
Western blot of human tissue lysates (15
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded breast tissue using anti-KRT19 mouse monoclonal antibody. (Clone UMAB3, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-KRT19 mouse monoclonal antibody. (Clone UMAB3, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB3, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB3, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded liver tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB3, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB3, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB3, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB3, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB3, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB3, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB3, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunofluorescent staining of MDCK cells
Immunofluorescent staining of MDCK cells using anti-CK19 mouse monoclonal antibody (UM500003, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
OriGene overexpression protein microarra
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-KRT19 mouse monoclonal antibody (Clone UMAB3). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-KRT19 (Clone UMAB3) very specifically recognizes KRT19 antigen on OriGene protein microarray chip.
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