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Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: UMAB2]

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Size:
100 µl _$$_
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Applications:

IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

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Product Description:

KRT19/CK19 mouse monoclonal antibody,clone UMAB2

Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CK19 monoclonal antibody (Clone UMAB2) at 1:500.
HEK293T were transfected with 55 differe
HEK293T were transfected with 55 different plasmids of CK cDNA (1, 2, 4, 5, 6a, 6b, 6c, 7, 8, 9, 12, 13, 14, 15, 16, 17, 18 v1, 18 v2, 19, 20, 24, 25, 26, 27, 28, 31, 32, 33a, 33b, 34, 35, 36, 37, 38, 39, 40, 71, 72 v1, 72 v3, 73, 74, 75, 76, 77, 78, 79, 80 v1, 80 v2, 81, 82, 83, 84, 85, 86 and 222) for 48hrs and lysed. Cell lysates (5 ug per lane) were separated by SDS-PAGE and blotted with KRT19 antibody. KRT12, 19, 25, 26, 27, 28 and 39 were positive, while all others were negative (1:500).
Western Blot analysis of 10 different hu
Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-CK19 monoclonal antibody (clone UMAB2, 1:500)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded breast tissue using anti-KRT19 mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-KRT19 mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded human liver tissue using anti-KRT19 mouse monoclonal antibody. Anti-KRT19 clone UMAB2 was diluted 1:100; used heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min to produce strong staining on the bile duct and no staining in the hepatocytes.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded lung tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-KRT19mouse monoclonal antibody. Clone UMAB2 was diluted 1:100; used heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min to produce strong staining on the exocrine glandular cells and no staining in the Islets of Langerhans
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Immunofluorescent staining of MCF7 cells
Immunofluorescent staining of MCF7 cells using anti-CK19 mouse monoclonal antibody (UM500002, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
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