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Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: UMAB1]

Catalogue number:
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UM500001
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
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Applications:

10k-ChIP, IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human, Monkey, Mouse, Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

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Product Description:

KRT8/CK8 mouse monoclonal antibody, clone UMAB1

OriGene overexpression protein microarra
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-KRT8 mouse monoclonal antibody (Clone UMAB1). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-KRT8 (Clone UMAB1) very specifically recognizes KRT8 antigen on OriGene protein microarray chip.
Immunofluorescent staining of A549 cells
Immunofluorescent staining of A549 cells using anti-CK8 mouse monoclonal antibody (UM500001, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody (Clone UMAB1) at 1:500.
HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8 (1:500).
Western blot of human tissue lysates (15
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
Western blot of mouse tissue lysates (20
Western blot of mouse tissue lysates (20ug) from Colon. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and KRT8-Knockout Hela cells (KO, Cat# LC810123) were separated by SDS-PAGE and immunoblotted with anti-KRT8 monoclonal antibody UM500001, (1:2000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-KRT8mouse monoclonal antibody. (Clone UMAB1, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
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