OxiSelect™ Cellular Antioxidant Assay Kit (Green Fluorescence)

Name: OxiSelect™ Cellular Antioxidant Assay Kit (Green Fluorescence)
Brand: Cell Biolabs
SKU: CBSKU_00535265
Price: 688 GBP
Availability: InStock

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STA-349

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Cell Biolabs

Size:

192 assays _$$_

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£688.00

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Estimated delivery Wed 24 Apr
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The OxiSelect™ Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells. Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

Data sheet: View or download

Storage Temperature:

Upon receipt, store the DCFH-DA Probe (1000X) and Quercetin at -20°C. Avoid multiple freeze/thaw cyc

Assay Principle for the OxiSelect™ Cellular Antioxidant Activity Assay Kit. An exogenous antioxidant compound is added to cells along with DCFH-DA dye. Upon entry into the cell, the DCFH-DA is cleaved to DCFH which can bind reactive oxygen species (ROS) generated within the cell by the addition of a free radical initiator. Binding of DCFH to ROS yields DCF, which produces a bright fluorescence. The presence of the exogenous antioxidant compound reduces the ROS available to the dye, yielding a lower fluorescent signal.

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