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OxiSelect™ Cellular Antioxidant Assay Kit (Green Fluorescence)

Supplier:
Catalogue number:
STA-349
Size:
192 assays
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£575.00 Estimated delivery Tue 28 Sep
Shipping is calculated in checkout

The OxiSelect™ Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells. Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

Product Type:

Biochemical and cellular assays

Storage Temp:

Upon receipt, store the DCFH-DA Probe (1000X) and Quercetin at -20°C. Avoid multiple freeze/thaw cyc

UNSPSC Code:

41116111

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