dopamine receptor-interacting protein 5, DRIP5, hRap1, RAP1, RAP1 homolog, repressor/activator protein 1 homolog, telomeric repeat-binding factor 2-interacting protein 1, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric protein 1, TRF2-in
between 100 and 150
2 - 8°C
Detection of human RAP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RAP1 antibody A303-532A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. RAP1 was also immunoprecipitated by rabbit anti-RAP1 antibodies A303-533A, which recognizes a downstream epitope, and A300-306A which is generated against full-length recombinant human RAP1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).