Nuclear poly(A)-binding protein 1, OPMD, PAB2, PABII, PABP2, PABP-2, poly(A) binding protein 2, poly(A) binding protein II, Poly(A)-binding protein 2, Poly(A)-binding protein II, Polyadenylate-binding nuclear protein 1, polyadenylate-binding protein 2
between 100 and 150
2 - 8°C
Detection of human PABPN1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PABPN1 antibody A303-524A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. PABPN1 was also immunoprecipitated by rabbit anti-PABPN1 antibody A303-523A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).