C-2k, CDC2L4, CDC2-related kinase, cell division cycle 2-like protein kinase 4, cell division protein kinase 9, CTK1, cyclin-dependent kinase 9, PITALRE, serine/threonine protein kinase PITALRE, Serine/threonine-protein kinase PITALRE, TAK, tat-associated
between 322 and 372
2 - 8°C
Detection of human CDK9 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CDK9 antibody A303-493A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. CDK9 was also immunoprecipitated by rabbit anti-CDK9 antibody A303-492A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).