50 kDa dynein-associated polypeptide, DCTN50, DCTN-50, dynactin 2 (p50), dynactin complex 50 kDa subunit, dynactin subunit 2, DYNAMITIN, epididymis secretory protein Li 77, epididymis secretory sperm binding protein, HEL-S-77, p50 dynamitin, RBP50
between 351 and 401
2 - 8°C
Detection of human DCTN2 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DCTN2 antibody A303-489A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. DCTN2 was also immunoprecipitated by rabbit anti-DCTN2 antibody A303-488A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).