LEF-1, lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF10, TCF1ALPHA, TCF1-alpha, TCF7L3
between 349 and 399
2 - 8°C
Detection of human LEF1 by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-LEF1 antibody A303-487A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. LEF1 was also immunoprecipitated by rabbit anti-LEF1 antibody A303-486A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).