activating signal cointegrator 1 complex subunit 3-like 1, ASCC3L1, bad response to refrigeration 2 homolog, BRR2, BRR2 homolog, HELIC2, RP33, small nuclear ribonucleoprotein 200kDa (U5), small nuclear ribonucleoprotein, U5 200kDa subunit, U5 small nuclea
between 400 and 450
2 - 8°C
Detection of human and mouse SNRNP200 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody A303-454A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. SNRNP200 was also immunoprecipitated by rabbit anti-SNRNP200 antibody A303-453A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).