C21orf112, Cctq, CCT-theta, chaperonin containing T-complex polypeptide 1 subunit 8, chaperonin containing TCP1, subunit 8 (theta), D21S246, PRED71, renal carcinoma antigen NY-REN-15, T-complex protein 1 subunit theta, TCP-1-theta
between 1 and 50
2 - 8°C
Detection of human and mouse CCT8 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CCT8 antibody A303-446A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. CCT8 was also immunoprecipitated by rabbit anti-CCT8 antibodies A303-447A, A303-448 and A303-449A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).